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| Column Chromatography |
Compounds are mixtures
of substances which are not tangible separately but collectively as the
compound whole. Separating the substances contained in the compound is called
chromatography which is one of the oldest methods to do so. The process of
chromatography involves adsorption of the slurry (mixture) in different
quantities. The mixture travels upwards and separation is achieved along the
way.
Silica gel column chromatography involves a glass tube filled with adsorbent to 1/3rd of its length. The tube is then introduced into a selective solvent leaving no air space in the tube the arrangement being called a ‘well packed column’. In this method the less polar component is washed out first. Also, there is a separation effected between the components completing the process of column chromatography.
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