Chromatography is said to be a technique which
is used to separate components
within any substance. It is basically a biological term with also helps to
calculate the chemical composition of any substance. This separation process
consists of two phases that is mobile and stationary phase. The basic working
is done on the stationary phase when adsorption is done and then the mobile
phase is passed into it. This is the procedure which helps in the separation of
the constituent mixtures. It is separated on the basis of its absorption rate
and solubility. Chromatography is of two types - absorption chromatography and
partition chromatography. Absorption chromatography is also divided into column
chromatography and thin layer chromatography. Partition chromatography is also
divided in to two types. They are paper and gas chromatography.
Among all the methods, people mostly prefer column
chromatography as it is the most convenient one and mostly used for the
separation of mixed organic substances. In case of silica gel column
chromatography, it is available in many types of requires sizes – 60-120 mesh,
30-200 mesh, 70-230 mesh, 100-200 mesh and many more. It is usually used for
the purifying the organic compounds like steroids, alkaloid and pharmaceutical
work as well.
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